Silencing of the prosystemin gene in the calluses of Physalis philadelphica (Solanaceae)
Plants respond to the attacks of herbivorous insects by activating the expression of genes involved in defense, which exert an effect on the development, reproduction and behavior of those insects. Sys- temin is an 18-amino acid peptide synthetized from the C-terminus of a protein precursor called pro- systemin that in tomato (Solanum lycopersicum L.) promotes the ex- pression of defense-related pro- teins that are crucial in resistance to herbivorous insects. Genetic analyses of the response to damage in tomato suggests that prosystem- in forms part of a defense signaling pathway that involves a complex regulation of the biosynthesis and perception of jasmonic acid (JA). In order to determine whether the mechanisms that regulate the defensive response in tomatillo (Physalis philadelphica Lam.) cor- respond to the signaling model posited for tomato, this study evaluated the functionality of the prosystemin in tomatillo during modulation of responses associ- ated with JA. To this end, silenced transgenic calluses were generated in the expression of prosystemin, in which the expression of marker genes of the signaling pathway of JA was analyzed.
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