Substrate and fertirigation for acclimatization of Fragaria x ananassa vitroplants

Substrate and fertirigation for acclimatization of Fragaria x ananassa vitroplants

Authors

  • Yutzil Duran Duran Tecnológico Nacional de México. Instituto Tecnológico del Valle de Oaxaca.
  • José Raymundo Enríquez Del Valle Tecnológico Nacional de México. Instituto Tecnológico del Valle de Oaxaca.
  • Vicente Arturo Velasco Velasco Tecnológico Nacional de México. Instituto Tecnológico del Valle de Oaxaca.
  • Maura Elisama Miguel Luna Tecnológico Nacional de México. Instituto Tecnológico del Valle de Oaxaca.
  • Fátima Manuel Zarate Tecnológico Nacional de México. Instituto Tecnológico del Valle de Oaxaca.

DOI:

https://doi.org/10.32870/e-cucba.vi22.347

Keywords:

Nutrient solution, strawberry micropropagation, substrates, preacclimatization

Abstract

The in vitro plant tissue culture technique is based on the isolation of organs, tissues or cells, which, by establishing them in aseptic conditions, and culture medium of defined composition and incubation, induce physiological responses, cell division and morphogenesis. An application of the tissue culture technique is micropropagation that consists of stages: 0) selection of the stock plant; I) surface disinfection of plant material to establish it in culture medium; II) multiplication of propagules; III) rooting of shoots, growth of seedlings and preparation for transplanting to substrate; IV) transfer of micropropagated plants to substrate for acclimatization in greenhouse. The objective was to evaluate the acclimatization of micropropagated strawberry Fragaria x ananassa Duch. plants that were established in substrates containing perlite and peat alone or combined in different proportions, and that were fertigated with different dilutions of nutrient solution. After 34 days of acclimatization from a total of 210 plants evaluated in the various substrate and fertigation conditions, all were acclimatized, and the plants that reached the biggest size were those established in substrates with 50 to 100% peat and were fertigated with any dilutions of nutrient solution.

References

Clavijo-Izquierdo, R., Beltrán-Castillo, A., Llauger-Riveron, R., Rodríguez-Dopazo, A., . Fanrrés -Armanteros, E., García-Álavez, M. y Rodríguez-Rubiai, M. (2010). Apuntes sobre el cultivo de la fresa (Fragaria x ananassa Duch.). CitriFrut, 27(2), 67-71.

Cruz-Crespo, E., Can-Chulim, A., Sandoval-Villa, M., Bugarin-Montoya, R., Robles-Bermúdez, A. y Juárez- López, P. (2012). Sustratos en la horticultura. Revista Bio Ciencias, 2(2), 17-26.

Enríquez-del Valle, J. R. (2008). La Propagación y Crecimiento de Agaves. Fundación Produce Oaxaca, A.C. e Instituto Tecnológico del Valle de Oaxaca. Oaxaca. México.

Gonzales-Arteaga, J.J., Rodríguez-Layza, J., Romero-Rivas, L.C., Párraga-Quintana, A. y Olivera-Soto, J.A. (2023). El rol del AIA y BAP en la regreneración y formación de brotes in vitro de tres variedades de fresa (Fragaria x ananassa Duch.). Agroindustrial Science, 13(2), 93-102. https://doi.org/10.17268/agroind.sci.2023.02.05

INEGI. Instituto Nacional de Estadística, Geografía e Informática (2005). Principales resultados por localidad. II Conteo de población y vivienda. http:// www.inegi.gob.mx/est/contenidos/esmand/conteo2005/ iter2005/selentcampo.aspx.

Mekonen, G., Chimdessa-Egigu, M. y Muthsuwamy, M. (2021). In vitro Propagation of Banana (Musa paradisiaca L.) Plant Using Shoot Tip Explant. Turkish Journal of Agriculture– Food Science and Technology, 9(12), 2339-2346. https://doi.org/10.24925/turjaf.v9i12.2339-2346.2883

Miguel-Luna, M E., Enríquez-del Valle, J.R., Velasco-Velasco, V.A., Campos-Ángeles, G.V. y J.L. Chávez-Servia. (2013). Acclimatization of micropropagated Musa cavendishii cultivar roatan plants submitted to doses of fertigation and auxin. African Journal of Agricultural Research, 8(43), 5336-5341.

Murashige, T. y Skoog, F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissues cultures. Physiologia Plantarum, 15, 473-497.

Neri, J. C., Meléndez-Mori, J.B., Tejada-Alvarado, J.J., Vilca-Valqui, N.C., Huaman-Huaman, E., Oliva, M. y Goñas, M. (2022). Optimized protocol for micropropagation and acclimatizacion of Strawberry (Fragaria x ananassa Duch.) Variety “Aroma”. Agronomy, 12 (4), 968. https://doi.org/10.3390/agronomy12040968

Romero-Rivas, L. C., González-Arteaga, J.J., Rodríguez-Layza, J., Párraga-Quintanilla, A. y Olivera-Soto, J.A. (2023). Multiplicación y enraizamiento in vitro de Fragaria x ananassa Duch. Var 'Aromas'. Revista de Investigaciones Altoandinas, 25 (4), 205-212. https://doi.org/10.18271/ria.2023.563

Secretaria de Agricultura y Desarrollo Rural (SADER). (2022). ¿Qué quiere la niña fresa? México y su producción nacional. https://www.gob.mx/agricultura/articulos/que-quiere-la-nina-fresa-mexico-y-su-produccion-nacional

Secretaria de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación, México. (SAGARPA). Fresa mexicana. Planeación Agrícola Nacional 2017-2023.https://www.gob.mx/cms/uploads/attachment/file/257075/Potencial-Fresa.pdf

Steiner, A.A. (1984) The Universal Nutrient Solution. Sixth International Congress on Soilless Culture, Wageningen, 633-650.

Valencia-Juárez, M., Escobedo-López, D., Díaz-Espino, L.F. y González-Pérez, E. (2019). Aclimatación ex vitro de plántulas de Fragaria x ananassa Duch. Revista Mexicana de Ciencias Agrícolas, 10(1), 91-100. https://doi.org/10.29312/remexca.v10i1.1633

Wafaa, A.F. y Wahdan, H.M. (2017). Influence of substrates on in vitro rooting and acclimatization of micropropagated strawberry (Fragaria x ananassa Duch.). Middle East Journal of Agriculture Research, 6(3), 682-691. https://www.curresweb.com/mejar/mejar/2017/682-691.pdf

Published

2024-05-01

How to Cite

Duran Duran, Y., Enríquez Del Valle, J. R., Velasco Velasco, V. A., Miguel Luna, M. E., & Manuel Zarate, F. (2024). Substrate and fertirigation for acclimatization of Fragaria x ananassa vitroplants: Substrate and fertirigation for acclimatization of Fragaria x ananassa vitroplants. E-CUCBA, (22), 54–60. https://doi.org/10.32870/e-cucba.vi22.347

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