Hypoglycemic effects of organic extracts from Ganoderma sp. and Kombucha in streptozotocin-induced diabetic Wistar rats
Hypoglycemic effects of organic extracts from Ganoderma sp. and Kombucha in streptozotocin-induced diabetic Wistar rats
DOI:
https://doi.org/10.32870/e-cucba.vi23.355Keywords:
streptomycin, insulin, islets of LangerhansAbstract
Evaluate the hypoglycemic effects of Kombucha and Ganoderma aqueous extracts in a model of diabetes with streptozotocin. Eight-week-ol male wistar rats weighimg approximately 180 g were randomized into four groups (12 rats each group). Control group (C), streptozotocin (E) without treatment, Kombucha (K) was given Kombucha extract 324 mg/kg orally, and Ganoderma group (G) was given 250 mg/kg orally both for 45 days. Four animals per group were sacrificed at 2-, 15- and 45-days post treatment. Consumption of water and food were registered. Blood glucose and insulin levels were measured by spectrophotometry and the islets of Langerhans in the pancreas were quantified by ELISA assay. Data were analyzed with an ANOVA two-ways and Tukey’s test as post hoc, Sigmastat 3.1 Software was used and 0.05% significance was considered. Among the main results found, both extracts presented hypoglycemic effects after 15 days of treatment. An interaction between the number of islets, insulin synthesis, water, and food consumption were also found with the streptozotocin group that presented the lowest average number of islets compared to other groups. Both extracts showed a hypoglycemic, protective and regenerative effect on the pancreas, with differences observed in the physical condition of the animals treated with Ganoderma, which showed signs of cachexia.
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Copyright (c) 2024 Pedro Hinojosa-Gómez , Dulce Anahí Pérez-Arvizo , Nancy Janette Márquez-Delgadillo, José Ricardo Cuéllar-Pérez, Guillermo Nolasco-Rodríguez , Lucía García-Delgado , Esther Albarrán-Rodríguez , Manuel Rosales-Cortés
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