Identificación del agente causal de la pudrición de raíz y corona en zarzamora cv.Tuppy y su control in vitro

Identification of the causal agent of the root and crown rot in blackberry cv. Tuppy and its in vitro control

Abstract

The disease known as blackberry root rot causes significant economic loses. The aim was to identify morphological and pathogenically fungus associated with root and crown rot in R. ulmifolius cv. Tuppy, and it’s in vitro control, from plants with symptoms of the disease collected in the common of San Juan de Dios, municipality of Los Reyes Michoacan. The causal agent was identified. The fungicides were used in three doses (low, medium, and high), Bacillus subtilis 0.075, 0.150, 0.225 ml L⁻¹, Captan® 0.0125, 0.025, 0.0375 g L⁻¹, Cymoxanil® 30% + Famoxadona® 0.015, 0.03, 0.045 g L⁻¹, Azoxystrobin®0.015, 0.03, 0.045 ml L⁻¹, Tiofanato Metílico® 0.025, 0.05, 0.075 g L⁻¹. The effectiveness of the product was evaluated with the growth mean of the fungus, the experimental design was completely randomized, and it was applied a Tukey mean comparison analysis at 5% probability of error. The virulence assessment and the severity of isolations was determined by pathogenicity tests in healthy plants. The phytopathogens identified were Rhizoctonia sp., and Fusarium oxysporum, all fungicides exercised in both phytopathogens to different degree. The pathogenicity tests were positive when presenting symptoms of the disease.