Characterization of strains of pleurotus djamor obtained by dicarion-monocaryon crossing
Characterization of strains of pleurotus djamor obtained by dicarion-monocaryon crossing
Keywords:
Mejoramiento genético, bagazo de agave, Fenómeno Buller, hongo comestibleAbstract
Buller phenomenon consists of the dikaryoticization of monokaryotic mycelia by dikaryonic mycelia, was first observed in Coprinus lagopus and Schizophyllum commune to determine incompatibility factors and understand the processes of nuclear migration and clamp formation, but it has not been used as a genetic improvement strategy in Pleurotus. The objective of the proposed work was to obtain and characterize di-monokaryonic strains of P. djamor native to Jalisco and their cultivation in tequila agave bagasse. To achieve this, the dikaryoticization of monosporic mycelia was studied by means of dikaryotic mycelia of three wild P. djamor strains from the state of Jalisco. A total of 9 di-monokaryonic strains were obtained. Each strain obtained was evaluated for its performance in EMA and HIT culture media, with the latter being the most suitable and therefore suitable for use in the management of these strains. Regarding agave carpophore production, it was determined that they can reach up to 39% biological efficiency in fresh and 4.9% in dry media. Furthermore, an average commercially suitable carpophore size of 5 to 11 cm was established, resulting in percentages ranging from 77% to 47% in the hybrid strains obtained.
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