Los metabolitos secundarios biosintetizados por las espinacas (Spinacea oleracea L.) y su cultivo in vitro: Una revisión
Secondary metabolites biosynthesized by spinach (Spinacea oleracea L.) and the in vitro culture: A review
DOI:
https://doi.org/10.32870/e-cucba.vi21.318Palabras clave:
Espinacetina, flavonoides, jaceidín, patuletina, 20-hidroxidisonaResumen
Las espinacas pertenecen a la familia taxonómica Amaranthaceae. Es una hortaliza de hoja anual y de rápido crecimiento considerada de alto valor nutritivo. Su producción internacional según estimaciones de la FAO fue de 14 millones de toneladas, siendo China el primer productor (85%), seguido por Estados Unidos (2.6%), Japón (2.2%) y Turquía (1.6%); mientras que, en México, en el año 2020 fue de 49,313 toneladas (+27.3% en comparación con 2019) obtenidas a partir de 2,853 ha cosechadas (+16.6%), por lo que el rendimiento promedio nacional fue de 17.3 ton/ha (+9.2%). Los principales constituyentes fitoquímicos de la espinaca predominantemente asociados a la calidad de las espinacas son los flavonoides totales, fenoles totales y carotenoides totales, debido a su actividad antioxidante (Bergquist, 2006). Entre sus valiosas propiedades medicinales destacan anticáncer, antimutagénica, antiinflamatorio, antiproliferativo, anticancerígeno, antibacteriano, hepatoprotector, hipolipidémicas y supresoras del Sistema Nervioso Central. El presente trabajo es una revisión sobre el origen, propiedades medicinales, perfil agronómico, perfil de metabolitos primarios y secundarios biosintetizados, cultivo in vitro de Spinacea oleracea y los datos sobre la composición fitoquímica de los metabolitos secundarios de las espinacas detectadas por medio de técnicas analíticas.
Citas
Abdelgawad, S.M., Hetta, M.H., Ibrahim, M.A. (2022). Phytochemical Investigation of Egyptian Spinach Leaves, a Potential Source for Antileukemic Metabolites: In Vitro and In Silico Study. Rev Bras Farmacogn, 32, 774–785. DOI: 10.1007/s43450-022-00307-0
Abdul-Wahab, F. y Abdul Jalil, T.(2012). Study of Iraqi Spinach Leaves (Phytochemical and Protective Effects Against methotrexate-Induced hepatotoxicity in rats). Iraqi J Pharm Sci, 21, 8-17.
Altemimi, A., Lakhssassi, N., Abu-Ghazaleh A. y Lightfoot, D.A. (2017). Evaluation of the antimicrobial activities of ultrasonicated spinach leaf extracts using rapd markers and electron microscopy. Arch Microbiol, 1–13, DOI: 10.1007/s00203-017-1418-6.
Bagheri, R., Bashir, H., Ahmad, J., M. Iqbal y Qureshi, M.I. (2015). Spinach (Spinacia oleracea L.) modulates its proteome differentially in response to salinity, cadmium and their combination stress. Plant Physiol Biochem, 97,235-45. DOI: 10.1016/j.plaphy.2015.10.012.
Bergman, M., Varshavsky, L., Gottlieb, H.E. y Grossman, S. (2001). The antioxidant activity of aqueous spinach extract: chemical identification of active fractions. Phytochemistry, 58,143-152.
Cai, X., Sun, X., Xu.,C., Sun, H., Wang, X. , Ge, C., Zhang, Z., Wang, Q., Fei, Z., Jiao, C. y Wang, Q. (2021). Genomic analyses provide insights into spinach domestication and the genetic basis of agronomic traits. Nature Communications. 12.
Das, S y Guha, D. (2008). CNS depressive role of aqueous extract of Spinacia oleracea L. leaves in adult male albino rats. Indian J Exp Biol, 46,185–90.
Geekiyanage, S., Takase, T., Watanabe, S., Fukai, S. y Kiyosue, T. (2006). The combined effect of photoperiod, light intensity and GA3 on adventitious shoot regeneration from cotyledons of spinach (Spinacia oleracea L.). Plant Biotechnol, 23, 431–435.
Hetta, M.H., Moawad, A.S., Hamed, M.A.A y Sabri, A.I. (2017). In-vitro and In-vivo hypolipidemic activity of spinach roots and flowers. Iran J Pharm Sci, 16,1509.
Howard, L.R., Pandjaitan, N., Morelock, T y Gil, M.I. (2002). Antioxidant capacity and phenolic content of spinach as affected by genetics and growing season. J. Agric. Food Chem, 50(21),5891-5896.
Hu, J. y Gao, J. (2020). Validation of a hplc method for flavoind contents in spina gleditsiae and its use to illustrate the various quality of cultivated varieties. Natural Product Communications, 15(8),193. DOI: 10.1177/1934578X20946258
Kabera, J., Semana, E., Mussa, A y He, X. (2014). Plant Secondary Metabolites: Biosynthesis, Classification, Function and Pharmacological Properties. J Pharma Pharm, 2, 377-392
Kidmose, U., Edelenbos, M. y Knuthsen, P. (2001). Carotenoids and flavonoids in organically grown spinach (Spinacia oleracea L) genotypes after deep frozen storage. Journal of the Science of Food and Agriculture, 81(9), 918-931.
Komai, F., Okuse, I. y Harada, T. (1996). Somatic embryogenesis and plant regeneration in culture of root segments of spinach (Spinacia oleracea L.). Plant Science, 113, 203-208. DOI:10.1016/0168-9452(95)04285-7
Leguillon, S., Charles, G. y Branchard, M. (2003). Plant regeneration from thin cell layers in Spinacia oleracea. Plant Cell, Tissue and Organ Culture, 74.
Li, Y., D. Kong., Y. Fu., M.R. Sussman y Wu, H. (2020)The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Phys Biochem. 148:80-89.
Lomnitski, L., Carbonatto, M., Ben-Shaul, V., Peano, S. y A. Conz (2000). The prophylactic effects of natural water-soluble antioxidant from spinach and apocynin in a rat model of lipopolysaccharide-induced endotoxemia. Toxicol Pathol, 28,588-600.
Murcia, M.A., Jiménez, M., Gonzalez, J y Martínez-Tomé, M. (2020). Spinach. En Nutritional Composition and Antioxidant Properties of Fruits and Vegetables. Academic Press,181-195.
Nyska, A., Suttie, A., Bakshi, S., Lomnitski, L. Grossman, S., Bergman, M., Ben-Shaul, V., Crocket, P., Haseman, J.K., Moser, G., Goldsworthy, R. y Maronpot, R. (2003). Slowing tumorigenic progression in TRAMP mice and prostatic carcinoma cell lines using natural anti-oxidant from spinach, NAO--a comparative study of three anti-oxidants. Toxicol Pathol, 31,39-51. doi: 10.1080/01926230390173833.
Nyska, A., Lomnitski, L., Spalding, J., Dunson, D.B., Goldsworthy, T.L., Ben-Shaul, V., Grossman, S., Bergman, M. y Boorman, G. (2001). Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model. Toxicol Lett, 122, 33-44. DOI: 10.1016/s0378-4274(01)00345-9.
Pandjaitan, N., Howard, L.R., Morelock, T. y Gil, M.I. (2005). Antioxidant capacity and phenolic content of spinach as affected by genetics and maturation. J. Agric. Food Chem, 53(22), 8618–23.
Roberts, J.L. y Moreau, R. (2016). Functional properties of spinach (Spinacia oleracea L.) phytochemicals and bioactives. Food & Function, 7(8), 3337-3353. DOI: 10.1039/c6fo00051g
Sara, A., Ulla, E.G., Knuthsen, P. y Olsson, M.E. (2005). Flavonoids in baby spinach (Spinacia oleracea L.): changes during plant growth and storage. J Agric Food Chem, 30;53(24), 9459-64. DOI: 10.1021/jf051430h.
Singh, A., Singh, P., Kumar, B., Kumar, S. y Maurya, R. (2019). Detection of flavonoids from Spinacia oleracea leaves using HPLC-ESI-QTOF-MS/MS and UPLC-QqQLIT-MS/MS techniques. Natural Product Research, 33(15), 2253-2256. DOI: 10.1080/14786419.2018.1489395
Van Nguyen, Q., Sun, H., Boo, K., Lee, D., Lee, J., Lim, P., Lee, H., Riu, K. y Lee, D. (2013). Effect of plant growth regulator combination and culture period on in vitro regeneration of spinach (Spinacia oleracea L.). Plant Biotechnology Reports ,7, 99–108.
Vázquez, E., García-Risco, M., Jaime, L., Reglero, G. y Fornari, T. (2013). Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products. J Supercrit Fluids, 82,138–45.
Xu, C., C. Jiao, H. Sun, X. Cai, X. Wang, C. Ge, Y. Zheng, W. Liu, X. Sun, Y. Xu, J. Deng, Z. Zhang, S. Huang, S. Dai, B. Mou, Q. Wang, Z. Fei y Q. Wang. (2017). Draft genome of spinach and transcriptome diversity of 120 Spinacia accessions. Nature Communications. 8.
Zhang, H., L. Lu, X. Zhao, S. Zhao, X. Gu, W. Du, H. Wei, R. Ji y L. Zhao. (2019). Metabolomics Reveals the "Invisible" Responses of Spinach Plants Exposed to CeO2 Nanoparticles. Environ Sci Technol, 21, 53(10), 6007-6017. DOI: 10.1021/acs.est.9b00593.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2024 Braulio Edgar Herrera Cabrera , Jorge Montiel Montoya , Rafael Salgado Garciglia, Luz María Basurto González, Andrés Carrillo del Rio, Bryan Jesús Vega Navarro, Hebert Jair Barrales Cureño
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.