Antibacterial Synergism of cepa Ganoderma sichuanese Gc73 and polyamines on gram-positive bacteria
Antibacterial Synergism of cepa Ganoderma sichuanese Gc73 and polyamines on gram-positive bacteria
DOI:
https://doi.org/10.32870/e-cucba.vi21.334Keywords:
Mycelial culture, fractionation, susceptible, streptomycinAbstract
During the last centuries, species of the Ganoderma lucidum complexhave been used in traditional Chinese medicine. "Lingzhi" has been used in Asia by their anticarcinogenic, immunomodulatory, antiviral and antibacterial properties. Taking into account a mycelium isolated from a live culture labeled as cepa G. sichuanense Gc73, the aim of this study is to determine the Minimum Inhibitory Concentrations (CIM) at which the culture of G. sichuanense cepa Gc73 broth supplemented with thiamine inhibits different strains of bacteria, both susceptible and resistant to streptomycin. Said culture broth after being centrifuged, filtered through a 0.22 µm pore membrane or separated by weight of <3,000 Daltons, showed synergy with PAs against different strains of bacteria. The results show an antibacterial effect against Bacillus subtilis ATCC (ATCC; Manassas, VA, USA) 6633, Staphylococcus aureus ATCC 6538 and Staphylococcus aureus ATCC 51811, Enterococcus faecalis ATCC 29212 and Micrococcus luteus ATCC 9341. The strains were tested. in concentrations of 2.5 to 50 µl of the culture broth supplemented with thiamin for each ml of Muller Hinton broth. We included six strains of Salmonella spp., previously isolated from raw meat, and the electrophoresis technique was used to determine the presence of antimicrobial peptides. The results showed that these peptides can only be identified in fresh samples of the extract.
References
Albino, E. F., Monache, F. D., Yunes, R. A., Paulert, R. y Junior, A. S. (2007). Antimicrobial activity of methyl australate from Ganoderma austral. Braz. J. Pharmacog, 17(1), 14-16.
Arenas, I., Villegas, E., Walls, O., Barrios, H., Rodríguez, R. y Corzo, G. (2016). Antimicrobial Activity and Stability of Short and Long Based Arachnid Synthetic Peptides in the Presence of Commercial Antibiotics. Molecules, 21, 225 DOI: 10.3390/molecules21020225
Baby, S., Johnson., A. J. y Govindan, B. (2015). Secondary metabolites from Ganoderma. Phytochemistry, 114, 66–101.
Cabarroi-Hernández, M., Decock, C., Welti, S., Amalfi, M., Villalobos-Arámbula, AR., Aliaga-Ramos, D., Morera, G., Macedo-Pérez Sandi, E., Almarales Castro, A. y Guzmán-Dávalos, L. (2023). Ganoderma from Cuba: an approach to some species based on morphology and phylogenetic analyses. Biological Journal of the Linnean Society, blad055. DOI: 10.1093/biolinnean/blad055
Chang, C. J, C. S. Lin, C.C. Lu, J. Martel, Y. F. Ko, D. M. Ojcius, S. F.Tseng, T. R. Wu, Y. Y. Chen, J. D. Young y H. C. Lai. (2015). Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun, 23. DOI: 10.1038/ncomms8489.
Chang, S. T. y Buswell, J. A. (1999). Ganoderma lucidum (lingzhi), a mushrooming medicinal mushroom. Int J Med Mushroom, 11, 1319–1346.
Chang, S. T. (2004). Ganoderma lucidum–a leader of edible and medicinal mushrooms. Int Agri Trade, 90, 22–24.
Choi, U. y Lee, C. R. (2019). Distinct Roles of Outer Membrane Porins in Antibiotic Resistance and Membrane Integrity in Escherichia coli. Front Microbiol, 30, 1-9. DOI: 10.3389/fmicb.2019.00953.
Ćilerdžić, J., Stajic, M. y Vukojevic, J. (2016). Potential of Submergedly Cultivated Mycelia of Ganoderma spp. as Antioxidant and Antimicrobial Agents. Curr Pharm Biotechnol, 17(3), 275-82.
De Silva, D. D., Rapior, S., Fons, F., Bahkali, A. H. et al. (2012). Medicinal mushrooms in supportive cancer therapies: an approach to anti-cancer effects and putative mechanisms of action. Fungal Diversity, 55, 1–35.
Demain, A. L. (2009). Antibiotics: natural products essential to human health. Med Res Rev, 29(6), 821-42.
Deng, H., Bloomfield, V. A., Benevides, J. M. y G. J. (2000). Structural basis of polyamine-DNA recognition: spermidine and spermine interactions with genomic B-DNAs of different GC content probed by Raman spectroscopy. Nucleic Acids Res. 1, 28(17), 3379-85.
Djide, M. N., Sartini, L. Rahman y N. Hasyim. (2014). Antibacterial Activity of Various Extracts From The Fruiting Bodies Of Ganoderma lucidum Growing At Samanea Saman (Jacq.) Merr) Trunk. Int J Sci & Technol Res, 3(1), 15-16.
Dong-Hun, B., Darius, J. R., Lane, P., Jansson, J. y Des, R. R. (2018). The old and new biochemistry of polyamines. Bbagen. DOI:10.1016/j.bbagen.2018.06.004.
Hadacek, F. y Bachmann, G. (2015). Low-molecular-weight metabolite systems chemistry. Front in Envir Sci Envir Toxicol. (3)12:1-21.
He, MQ., Zhao, RL., Liu, DM., Denchev, TT., Begerow, D., Yurkov, A., Kemler, M., Millanes, AM., Wedin, M., McTaggart, AR., Shivas, RG., Buyck, B., Chen, J., Vizzini, A., Papp, V., Zmitrovich, IV., Davoodian, N., Hyde, KD. (2022). Species diversity of Basidiomycota. Fungal Diversity, 114, 281–325. DOI: 10.1007/s13225-021-00497-3
Igarashi, K. y Kashiwagi, K. (2010). Characteristics of cellular polyamine transport in prokaryotes and eukaryotes. Plant Physiol and Biochem. 48. 506e512.
Igarashi, K., Kashiwagi, K. (2010). Characteristics of cellular polyamine transport in prokaryotes and eukaryotes. Plant Physiol Biochem, 48(7), 506-512. DOI: 10.1016/j.plaphy.2010.01.017.
Isaka, M., Chinthanom, P., Kongthong, S. y Srichomthong, K. (2013). Lanostane triterpenes from cultures of the Basidiomycete Ganoderma orbiforme BCC 22324. Phytochemistry, 87, 133–139.
Jiao-Jiao, Z., Dai-Wei, W., Dan, C., Qing, L. y Yong-Xian, Ch. (2021). Meroterpenoids From Ganoderma lucidum Mushrooms and Their Biological Roles in Insulin Resistance and Triple-Negative Breast Cancer. Front Chem, 9. DOI: 10.3389/fchem.2021.772740.
Osińska-Jaroszuk, M., Jaszek, M., Mizerska-Dudka, M., Błachowicz, A., Rejczak., TP, Janusz, G., Wydrych, J., Polak, J., Jarosz-Wilkołazka, A., Kandefer-Szerszeń, M. (2014). Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties. Biomed Res Int. DOI: 10.1155/2014/743812.
Jong, S. C. y Birmingham, J. M. (1992). Medicinal benefits of the mushroom Ganoderma. Adv Appl Microbiol, 37, 101–34.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
Loyd, A. L., Richter, B. S., Jusino, M. A., Truong, C., Smith, M. E., Blanchette, R. A. y Smith, J. A. (2018). Identifying the “Mushroom of Immortality”: Assessing the Ganoderma Species Composition in Commercial Reishi Products. Front. Microbiol. 9, 1557, 1-14. DOI: 10.3389/fmicb.2018.01557
Martínez-Chávez, L., Cabrera-Diaz, E., Pérez-Montaño, J. A., Garay-Martínez, L. E., Varela-Hernández, J. J., Castillo, A., Lucia,L., Ávila-Novoa, M. G., Cardona-López, M. A., Gutiérrez-González, P. y Martínez-Gonzáles, N. E. (2015). Quantitative distribution of Salmonella spp. and Escherichia coli on beef carcasses and raw beef at retail establishments. Int J Food Microbiol, 1(210), 149-155.
Martini, C., Michaux, C., Bugli, F., Arcovito, A., Iavarone, F., Cacaci, M., Paroni Sterbini, F., Hartke, A., Sauvageot, N., Sanguinetti, M., Posteraro, B. y Giard, J. C. (2015). The polyamine N-acetyltransferase-like enzyme PmvE plays a role in the virulence of Enterococcus faecalis. Infect Immun, 83(1), 364-371.
Mojsoska, B. y Jenssen, H. (2015). Peptides and Peptidomimetics for Antimicrobial Drug Design. Pharmaceuticals, 8, 366-415.
Neuhoff, V., Arold, N., Taube, D. y Ehrhardt, W. (1988). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis, 9, 255-262.
Ofodile, L. N., Wanneka, A. O. y Oladipupo, O. (2011). Effect of the Mycelial Culture of Ganoderma lucidum on Human Pathogenic Bacteria. Int J of Biol, 3(2), 111-114.
Ofodile, L. N. (2006). Taxonomy and Antimicrobial Activity of some Basidiomycetous Fungi in Southern Nigeria. PhD Thesis. Department of Botany and Microbiology, University of Lagos. Akoka, Lagos: 6-44.
Paterson, R. R. M. (2006). Ganoderma a therapeutic fungal biofactory. Phytochemistry, 67(18), 1985–2001.
Sanodiya, B. S., Thakur, G. S., Baghel, R. K., Prasad, G. y Bisen, P. S. (2009). Ganoderma lucidum: A Potent Pharmacological Macrofungus. Curr Pharm Biotech, 10(8), 717-742.
Sarnthima, R., Khammaung, S. y Sa-Ard, P. (2017). Culture broth of Ganoderma lucidum exhibited antioxidant, antibacterial and α-amylase inhibitory activities. J Food Sci Technol, 54(11), 3724-3730. DOI: 10.1007/s13197-017-2839-6.
Si, J., Meng, G., Wu, Y., Ma, HF., Cui, BK., Dai, YC. (2019). Medium composition optimization, structural characterization, and antioxidant activity of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi. International Journal of Biological Macromolecules, 124, 1186-1196. DOI: 10.1016/j.ijbiomac.2018.11.274
Suhnel, S., Lagreze, F., Ferreira, J. F., Campestrini, L. H. y Maraschin, M. (2009). Carotenoid extraction from the gonad of the scallop Nodipecten nodosus (Linnaeus, 1758) (Bivalvia: Pectinidae). Braz. J. Biol, 69(1), 209-215.
Tan, B. K. H. y Vanitha, J. (2004). Immunomodulatory and antimicrobial effects of some traditional Chinese medicinal herbs: a review. Curr Med Chem. 11: 1423–1430.
Valentão, P., Fernandes, E., Carvalho, F., Branquinho, P., Seabra, R. y M. Bastos. (2002). Studies on the Antioxidant Activity of Lippia citriodora Infusion: Scavenging Effect on Superoxide Radical, Hydroxyl Radical and Hypochlorous Acid. J Biol. Pharm. Bull, 25(10), 1324-1327.
Vazirian, M., Faramarzi, M. A., Ebrahimi, S. E., Esfahani, H. R., Samadi, N., Hosseini, S. A., Asghari, A., Manayi, A., Mousazadeh, A., Asef, M. R., Habibi, E. y Amanzadeh, Y. (2014). Antimicrobial effect of the Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes) and its main compounds. Int J Med Mush, 16(1), 77-84.
Wachtel-Galor, S., Yuen, J., Buswell, J. A. y Benzie, I. (2011). Ganoderma lucidum (Lingzhi or Reishi) A Medicinal Mushroom. Major bioactive components; Triterpenes, in: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. (2da edición). Boca Raton (FL): CRC Press/Taylor & Francis.
Wang, H. y Ng, T. B. (2012). Ganodermin, an antifungal protein from fruiting bodies of the medicinal mushroom Ganoderma lucidum. Peptides, 27(1), 27–30.
Wasser, S. P. y Weis, A. L. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives (Review). Int J Med Mushrooms, 1, 31–62.
Yihuai, U., Zhau, S., Huang, M. y Xu, A. (2003). Antibacterial and Antiviral Value of the Genus Ganoderma Species (Amphyllophoro mycetideae): A Review. Int J Med Mushrooms, 5, 235-246.
Zuluaga, J., Pérez, C., Angulo, A., Torres, O. y Santafé, G. (2007). Química y actividades antioxidante y bactericida del extracto etanólico del hongo Ganoderma lucidum. Scientia Et Technica, (33), 329-332.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Ramón Reynoso-Orozco, Luis Alberto Barajas-Villegas, Sergio Fausto-Guerra, Cecilia Jiménez Plascencia, Teresa de Jesús Jaime Ornelas, Benito Donato Minjarez Vega
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
