Sinergismo antibacterial de la cepa G. sichuanense Gc73 y poliaminas en bacterias gram-positivas
Antibacterial Synergism of cepa Ganoderma sichuanese Gc73 and polyamines on gram-positive bacteria
DOI:
https://doi.org/10.32870/e-cucba.vi21.334Palabras clave:
Cultivo micelial, fraccionamiento, susceptibles, estreptomicinaResumen
Durante los últimos siglos, las especies del complejo Ganoderma lucidum se han utilizado en la medicina tradicional china. “Lingzhi” ha sido usado en Asia por sus propiedades anticancerígenas, inmunomoduladoras, antivirales y antibacterianas. Teniendo en cuenta un micelio aislado de un cultivo vivo etiquetado como la cepa G. sichuanense Gc73, el objetivo de este estudio es determinar las Concentraciones Inhibitorias Mínimas (CIM) a las que se usa el caldo de cultivo de G. sichuanense cepa Gc73 suplementado con tiamina para inhibir diferentes cepas de bacterias, tanto sensibles como resistentes a la estreptomicina. Dicho caldo de cultivo tras ser centrifugado, filtrado a través de una membrana de poro de 0,22 µm o separado por peso de < 3.000 Daltons, demostró la sinergia con PAs contra diferentes cepas de bacterias. Los resultados muestran un efecto antibacteriano frente a los Bacillus subtilis ATCC (ATCC; Manassas, VA, EE. UU.) 6633, Staphylococcus aureus ATCC 6538 y Staphylococcus aureus ATCC 51811, Enterococcus faecalis ATCC 29212 y Micrococcus luteus ATCC 9341. Las cepas fueron probadas en concentraciones de 2.5 a 50 µl del caldo de cultivo suplementado con tiamina por cada ml de caldo Muller Hinton. Incluimos seis cepas de Salmonella spp., previamente aisladas de carne cruda y se utilizó la técnica de electroforesis para determinar la presencia de péptidos antimicrobianos. Los resultados mostraron que estos péptidos solo pueden identificarse en muestras frescas del extracto.
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Derechos de autor 2024 Ramón Reynoso-Orozco, Luis Alberto Barajas-Villegas, Sergio Fausto-Guerra, Cecilia Jiménez Plascencia, Teresa de Jesús Jaime Ornelas, Benito Donato Minjarez Vega
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
